Lab4 and Cholesterol Study

The anti-cholesterolaemic effect of a consortium of probiotics: An acute study in C57BL/6J mice (Part 1)

SUMMARY

This double-blind, placebo-controlled study examines the role of probiotic administration in the prevention of C. difficile-associated diarrhoea (CDAD) in elderly patients receiving antibiotic therapy.​
Results showed that Lab4 probiotics reduced the incidence of Clostridium difficile diarrhoea in these patients.​
46% of patients supplemented with Lab4 probiotics were positive for Clostridium difficile toxin compared to 78% of patients in the placebo group

Aim

To examine the cholesterol lowering abilities of Lab4 probiotics in an in vitro model of the intestinal epithelium.

Method

• Cholesterol lowering ability and bile salts hydrolase (BSH) activity in Lab4 probiotics was assessed using in vitro models
• The effect of Lab4 probiotics on cholesterol transport was assessed in an in vitro Caco-2 cells intestinal model

Results

Cholesterol removal

• Cholesterol levels in bacterial culture media were lowered in the presence of Lab4 probiotic mixture (#P=0.076)

Bile salt hydrolase activity

• A white precipitate was observed when Lab4 probiotic mixture was grown in the presence of bile salts, which is indicative of BSH activity.

Cholesterol transport

• Lab4 probiotic mixture significantly reduced the expression of cholesterol transporter NPC1L1, which is critical for the uptake of cholesterol (**P<0.001)

• Lab4 probiotic mixture significantly reduced the efflux of intracellular cholesterol from intestinal epithelial cells into the basolateral (tissue) compartment (*P=0.004)

• Lab4 probiotic mixture significantly reduced the expression of cholesterol transporter ABCA-1, which is involved in the efflux of intracellular cholesterol to the basolateral (tissue) compartment (*P<0.01)

Summary Proposed Mechanism of Action

1. CHOLESTEROL REMOVAL [cholesterol is bound or metabolised by Lab4 and subsequently excreted in faeces]
2. BILE SALT HYDROLASE ACTIVITY [deconjugation (modifying) of bile acids leads to their reduced re-absorption and places an increased demand on the liver to synthesize more bile acids from circulating cholesterol in blood to replenish intestinal bile acids lost in faeces]
3. REDUCTION OF CHOLESTEROL TRANSPORT ACROSS THE INTESTINAL EPITHELIUM [3a – reduced expression of the NCP1L1 cholesterol transporter; 3b- reduction in the efflux of intracellular cholesterol to the basolateral compartment (tissue) and reduced expression of the ABCA-1 cholesterol transporter; 3c – no change on the intracellular cholesterol efflux back into the apical (intestinal lumen) compartment]

Conclusion

Lab4 probiotics showed promising cholesterol lowering potential.

Reference

Michael DR et al. 2017 The anti-cholesterolaemic effect of a consortium of probiotics: An acute study in C57BL/6J mice. Scientific Reports 7:2883
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